Immunohistochemistry (IHC) is a powerful technique to study the presence and location of a specific protein in a tissue. The success of this method relies upon the highly specific antibody-antigen interaction and its detection. Since 1940 when the technique was first described (Coons, 1940), the ICH protocol had undergone several improvements such as the increased sensibility of the detection system. IHC provides crucial information for several applications by making it possible to visualize the distribution of specific cellular components within cells and in the proper tissue. Therefore, it is widely use in diagnostic to determine morphological abnormalities and to the detection of stained markers indicative of certain diseases. As a result of technological progress, there are multiple approaches and permutations in ICH methology. The antigen-antibody choice, the tissue fixation methods, the immunohistochemistral markers and microscopy are steps that may vary accordingly to the research goals. Thus, there are an enormous variety of possibilities which led to a great range of protocols suitable for the most diverse proposes. Here in the website we have focus in IHC staining of formalin-fixed and paraffin-embedded tissues (FFPE) by using the Labeled Streptavidin-Biotin Complex (LSAB) method.
Applications: Cancer diagnostic, prognostic and therapeutical decisions. Diagnostic of Infeccious Diseases. Research in diverse fields. |